View statistics for this project via Libraries. Note that this requires Cython to build the sources, see the build instructions on the project home page.
Note that this requires Cython to be installed at an appropriate version for the build. Nov 2, Mar 21, Nov 27, Oct 18, Oct 17, Jul 9, May 19, Jan 29, Nov 25, Aug 11, Jul 27, Jun 10, Mar 26, Feb 28, Feb 9, Jan 4, Jan 3, Sep 10, Aug 3, Jun 27, Jun 22, Mar 13, Nov 4, Oct 13, Sep 17, Jun 3, Feb 18, Jan 8, Dec 23, Dec 10, Aug 20, Aug 18, Jul 24, Mar 17, Nov 13, Sep 18, Apr 25, Apr 15, Feb 7, Nov 20, Aug 28, Apr 18, Apr 3, Mar 4, Many measurements that were previously limited to cuvette type studies are now being incorporated into fluorescence microscopes and into medical diagnostics such as the endoscope.
A brief general description of the various methods in our laboratory will be presented. The reason for making fluorescence lifetime measurements in images will be discussed. The necessity for real-time imaging is stressed, and the performance characteristics of our latest fast-FLI instrument will be briefly explained including methods for rapid, informative display of complex data. The unique information available will be emphasized. We will also discuss measurements of two-photon excitation imaging in tissue.
Recent results of quantitative determinations of reactive oxygen species caused by sun exposure in biological tissue will be presented. Early detection of fruit skin storage diseases like bitterpit and storage scald is aided by macroscopic imaging of healthy and diseased tissue. Fast, whole area detection is necessary for quickly assessing fruit quality and for predicting storage potential. Macroscopic fluorescence lifetime imaging enhances contrast w. By monitoring photosystem II Chlorophyll a fluorescence it provides quick physiological information over an area not practically accessible by confocal TPE microscopy Biophys.
Here we report using a fast-acquisition homodyning phase and modulation imaging fluorimeter Biophys. Operating frequency was Time progression of total intensity, phase and modulation images of diseased and healthy tissue are presented. Differences in observed Chlorophyll fluorescence lifetimes range from 0. Kautsky-effect fluorescence induction effects on imaging are discussed. Fluorescence lifetime-resolved imaging FLI provides indispensable, valuable information than is not available from steady-state fluorescence image measurements.
In general, because of the complexity of the analysis compared to the display of simple intensity images, FLI measurements have required much longer times to process and display. However, many biological and medical applications using fluorescence imaging require real time acquisition, processing and display.
Real-time operation is necessary for following dynamic biological events, or carrying out medical diagnostics. We have developed a real-time FLI system for a variety of imaging applications. The instrument uses rapid frequency-domain data acquisition hardware; however, here we demonstrate software that specifically enables the real-time processing and highly informative, convenient and easy-to-understand display of the lifetime-resolved fluorescence information.
A menu of possibilities is provided to the operator to assist in the on-line interpretation and control of real-time experiments and rapid events. The programs have been streamlined in order to make the multiple-parameter, lifetime-resolved information available to the user, and provide a continual up-to-date view of the statistics.
Reinhardtii was observed with a new high-speed instrument for measuring fluorescence lifetime-resolved microscope images.
Objects are imaged using the frequency domain phase and modulation technique in homodyne mode. The laser light is modulated at a high frequency. The fluorescence image is focused onto a modulated image intensifier and the phase-resolved images are captured on a fast CCD camera, sequentially transferred to a PC computer and displayed in real-time.
Rapid measurements are necessary during the fluorescence transient maximum the P-level following initial illumination. The observed lifetime heterogeneity is in accordance with photosynthetic functionality. Time-resolved fluorescence microscopy of the NPQ mutants is expected to provide new information on the role of the Xanthophyll cycle in the non-photochemical quenching process in single algal cells.
The chlorophyll a Chl a fluorescence from single cells of wild type WT and non-photochemical quenching NPQ mutants NPQ1 and NPQ2 of Chlamydomonas reinhardtii was observed with a new high-speed instrument for measuring fluorescence lifetime-resolved images see Instrumentation.
Fluorescence transient studies see poster by Seufferheld et al. The time-resolved fluorescence microscopy of the NPQ mutants can therefore be used to study the role of the Xanthophyll cycle in the non-photochemical quenching process in single cells. We present a new high-speed instrument for rapidly measuring fluorescence lifetime-resolved images of large as well as small objects in real time up to 55 phase-resolved images per second. This instrument employs stage-scanning while measuring lifetime-resolved fluorescence images in the phase and modulation, homodyne mode.
Long working-distance objectives 5 to times magnification are used for illuminating the sample with HF-modulated laser light; the sample is placed on a piezo-controlled xy-scanning stage; all pixels of the fluorescence image are imaged simultaneously and projected onto a HF-modulated image intensifier. A fast CCD camera captures the incrementally phase-delayed modulated images, that are acquired, processed and displayed in real-time on a PC computer.
Light modulation of the CW laser is achieved with a standing wave acousto optical modulator. We demonstrate the functionality and use of the instrument with different examples. The instrument is especially useful for rapidly scanning even larger extended objects while simultaneously displaying lifetime-resolved images as rapid as or faster than video mode.
Such rapid data acquisition is also essential for measuring lifetime-resolved images that are rapidly changing their fluorescence characteristics due to e.
Purpose: To determine the photophysical properties of tetramethylrhodamine TMRh conjugated to single-stranded ss and doublestranded ds DNA oligomers. Methods: TMRh was coupled via a C6 linker to the 5' end of oligonucleotides. If downloads fail, reload this page, enable JavaScript, disable download managers, disable proxies, clear cache, use Firefox, reduce number and frequency of downloads.
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